DNA Gyrase/DHFR-IN-1 is a dual inhibitor targeting bacterial DNA gyrase and DHFR, with IC50 values of 182 μM and 3.90 μM against E. coli DNA gyrase and DHFR, respectively. This compound exhibits both bactericidal and antifungal activities.

DNA Gyrase/DHFR-IN-1 的空间阻隔抗性水平分别显示为：枯草芽孢杆菌 16 nM、金黄色葡萄球菌 24 nM、大肠杆菌 51 nM、铜绿假单胞菌 53 nM、黄曲霉 15.50 μM 和白色念珠菌 19.50 μM。