DNA crosslinker 2 (dihydrochloride) is a potent minor groove binder exhibiting a Δ T m of 1.2 °C, indicative of its DNA binding affinity. This compound demonstrates inhibitory activity against cancer cell lines NCI-H460, A2780, and MCF-7, making it applicable for anticancer research [1].

DNA交联剂2（二盐酸盐）（化合物3）在100微摩尔浓度下，处理时间48小时或96小时，展现了对NCI-H460、A2780和MCF-7细胞[1]的抑制活性。