Disperse Yellow 54 is a multifunctional dye essential in biological experiments, aiding in the observation and analysis of cell structures, tracking of biomolecules, evaluation of cell functions, distinguishing cell types, detection of biomolecules, study of tissue pathology, and monitoring of microorganisms. Its applications span basic scientific research to clinical diagnostics, as well as traditional fields like textile dyeing and emerging areas such as functional textile processing, food pigments, and dye-sensitized solar cells.