DiI iodide1 is one of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water.

Stain adherent cells: Grow adherent cells on sterile glass coverslips. Remove coverslips from growth medium and gently drain off excess medium. Place coverslip in a humidity chamber. Pipet 100 μL of the dye working solution onto the corner of a coverslip and gently agitate until all cells are covered. Incubate the coverslip at 37℃ for 2-20 minutes. Drain off the dye working solution and wash the coverslips two to three times with pre-warmed growth medium. Microscopy Detection: DiI, 549/565 nm, XF32-Omega, 31002-Chroma; DiR, 748/780 nm, XF112-Omega, 41009-Chroma