Cystathionine β-lyase, an enzyme crucial for bacterial virulence [1], catalyzes the conversion of cystathionine into homocysteine, representing the penultimate step in the biosynthesis of methionine.

Cystathionine β-lyase（25 nM；10 分钟）催化 L-(+)-胱氨酸（100 μM）转化为同型半胱氨酸。其活性通过使用 5,5′-二硫偶氮二苯酸（DTNB）在 412 nm 处对游离巯基的测量来监测同型半胱氨酸的产生[1]。