Collagenase, Type VIII, has the capability to hydrolyze Type VIII collagen, and may be utilized in the study of atherosclerosis development. Type VIII collagen serves as a regulator of endothelial cell differentiation and angiogenesis, acts as a substrate for the adhesion and migration of cells such as smooth muscle cells, and may accumulate in atherosclerosis. Following the activation by endotoxins, the expression of Collagenase, Type VIII can reduce the production of Type VIII collagen, potentially inhibiting the progression of atherosclerosis.