Chol-N3 is a bioorthogonal chol probe capable of integrating with super-resolution fluorescence microscopy (SRM) to enable direct nanoscale imaging of lipid heterogeneity on resting living cell surfaces [1]. As a click chemistry reagent with an azide group, Chol-N3 undergoes copper-catalyzed azide-alkyne cycloaddition (CuAAc) with alkyne-bearing molecules and can also participate in strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO or BCN group-containing molecules.

Chol-N3（25 μM; 16小时; 活的SH-SY5Y细胞）在维持细胞活性的实验条件下显示，该化合物在质膜（PM）中明确揭示了纳米尺度的结构域分布情况[1]。