CHIKV-IN-5 (Compound 26) is an inhibitor of CHIKV with an EC90 value of 0.45 μM. It suppresses the replication of CHIKV in the later stages of the viral life cycle by blocking the translation of structural proteins. CHIKV-IN-5 demonstrates high stability in vitro within mouse microsomes. In infected mice, it alleviates footpad swelling and reduces the spread of the virus to other tissues.

CHIKV-IN-5 在正常人真皮成纤维细胞中以10 μM浓度显示出良好的病毒滴度降低效果（VTR=8.7 log），并且在体外小鼠微粒体中具有稳定性（MLM t1/2 of 74 min）。CHIKV-IN-5（10 μM, 2小时）可阻止CHIKV进入NHDF细胞后的复制。此外，预处理1小时的CHIKV-IN-5（10 μM）能抑制CHIKV感染的NHDF细胞中CHIKV结构蛋白的合成，但不影响病毒转录。

CHIKV-IN-5（80 mg/kg，腹腔注射，每天两次，持续 3 天）能够减轻 CHIKV 感染小鼠的足垫肿胀，并显著抑制病毒蔓延至其他组织。CHIKV-IN-5 (0.5-20 mg/kg，通过腹腔、静脉、口服及皮下注射) 在雄性 C57BL/6 小鼠中展现出良好的代谢稳定性。