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CGamF是一种荧光探针,主要用于研究肝脏中的胆酸运输。此探针由胆酸(货号20250)与荧光素通过甘氨酸连接基耦合而成。在pH 7.4条件下的水溶液中,CGamF的激发和发射峰值分别是492 nm和515 nm。值得注意的是,在495 nm的激发波长下,其荧光强度会随着pH的增加而增强;然而在440 nm激发下,荧光强度却不受pH影响。此外,CGamF的肝细胞吸收随探针浓度增加而增强,且此过程在低钠环境下会受到抑制。CGamF已广泛应用于体外研究, 用以探讨肝脏转运蛋白介导的药物相互作用和毒性。
CGamF是一种荧光探针,主要用于研究肝脏中的胆酸运输。此探针由胆酸(货号20250)与荧光素通过甘氨酸连接基耦合而成。在pH 7.4条件下的水溶液中,CGamF的激发和发射峰值分别是492 nm和515 nm。值得注意的是,在495 nm的激发波长下,其荧光强度会随着pH的增加而增强;然而在440 nm激发下,荧光强度却不受pH影响。此外,CGamF的肝细胞吸收随探针浓度增加而增强,且此过程在低钠环境下会受到抑制。CGamF已广泛应用于体外研究, 用以探讨肝脏转运蛋白介导的药物相互作用和毒性。
产品描述 | CGamF is a fluorescent probe designed for bile acid transport, consisting of a bile acid (Product No. 20250) linked to fluorescein via a glycine connecting group. In aqueous solution at pH 7.4, the excitation/emission wavelengths of this probe are 492/515 nm, respectively. The fluorescence intensity of CGamF increases with rising pH when excited at 495 nm, while the intensity is unaffected by pH changes when excited at 440 nm. Sodium-dependent uptake of CGamF by hepatocytes increases with the concentration of the compound, with reduced absorption at lower sodium levels. CGamF has been utilized in vitro to study drug interactions and toxicity mediated by hepatic transporters. |
别名 | FITC-glycocholate, FITC-GC, Cholylglycylamidofluorescein |
分子量 | 794.9 |
分子式 | C46H54N2O10 |
CAS No. | 168912-70-7 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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