CelGreen nucleic acid gel stain *10,000× concentrate in water* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal.

GelGreen用于凝胶电泳染色（预染色，方法同EB）。首先准备琼脂糖溶液并加热至溶解。GelGreen核酸染色溶液需从GelGreen核酸染色剂按1:10,000稀释后再加入琼脂糖溶液。由于GelGreen具有良好的热稳定性，可以直接加入热的琼脂糖溶液中。确保充分混匀后倒制凝胶，进行电泳并使用UV投影仪查看结果。