CDK7-IN-30 (Compound 22) is an inhibitor of CDK7 with an IC50 value of 7.21 nM, effectively inhibiting the phosphorylation of RNA polymerase II and CDK2. It can induce cell apoptosis (apoptosis) and exhibits anticancer activity, making it useful for cancer research.

CDK5:3678.50 nM, CDK4:644.80 nM, CDK1:>10000.00 nM, CDK7:7.21 nM, CDK2:734.55 nM, CDK3:370.40 nM, CDK9:704.3 nM, CDK6:1345.50 nM

CDK7-IN-30(化合物22)在多种癌细胞中显示出显著的抗增殖效应,其IC 50 分别为对RS4;11细胞288.0 nM、MM.1S细胞174.6 nM、Mino细胞37.5 nM与Jeko-1细胞168.7 nM.此外,该化合物对正常血细胞PBMCs的影响相对较小,IC 50 值为757.9 nM.在MV4-11细胞中,CDK7-IN-30(50-500 nM; 24 h)能引起细胞周期在G1/S阶段的停滞,并且在更高浓度(100-1000 nM; 48 h)时促使细胞发生凋亡.