C.I. Acid Blue 158 is a multifunctional dye used in biological experiments to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Its applications range from basic scientific research to clinical diagnostics. Additionally, dyes like C.I. Acid Blue 158 are widely used in traditional fields such as textile dyeing, as well as in emerging fields like functional textile processing, food pigments, and dye-sensitized solar cells.