BODIQPy-TPA is a lipophilic probe that induces ferroptosis in B16 and HepG2 cells upon light irradiation through lipid peroxidation. It exhibits a maximum absorption wavelength of 488 nm and a maximum emission wavelength above 640 nm [1].

BODIQPy-TPA (5 μM) 在蓝光 LED 照射下对 B16 细胞显示出 ferrostatin 依赖性的光毒性，IC 50 为 0.51 μM，并且下调 ferritin heavy polypeptide 1 (FTH1) 的表达 [1]。BODIQPy-TPA (5 μM) 通过光照射增加三酰甘油 (TG) 水平，并下调磷脂酰胆碱 (PC) 的水平 [1]。Cell Viability Assay [1] Cell Line: B16 Concentration: 0-50 μM Incubation Time: 48 h Result: 抑制 B16 细胞活力，在 ferrostatin 存在下细胞毒性降低。Western Blot Analysis [1] Cell Line: B16 Concentration: 5 μM Incubation Time: 48 h Result: FTH1 水平下降。