AV123 (compound 12) is a non-cytotoxic RIPK1 inhibitor with IC 50 of 12.12 μM. AV123 blocks the TNF-α-induced necroptotic with EC 50 of 1.7 μM, but not the apoptotic cell death. AV123 can be used in the study of necrotic chronic conditions such as ischemia-reperfusion injury of the brain, heart and kidney, inflammatory diseases, neurodegenerative diseases and infectious diseases [1].

AV123 (0.01-100 μM; 24 h) efficiently blocks necroptosis in a dose-dependent manner in FADD-deficient Jurkat cells [1]. AV123 (0.01-50 μM; 24 h) is not toxic to RPE-1 cells [1]. Cell Viability Assay [1] Cell Line: FADD-deficient Jurkat cells Concentration: 0.01-100 μM Incubation Time: 24 h Result: Significantly blocked the necroptotic cell-death induced by TNF-α with an EC 50 value of 1.7 μM. Cell Cytotoxicity Assay [1] Cell Line: RPE-1 cells Concentration: 0.01-50 μM Incubation Time: 24 h Result: Exhibited no toxicity to RPE-1 cells.