Antioxidant agent-3 (Compound 14q), a potent antioxidant, exhibits strong scavenging activity against DPPH radicals and ABTS+ radicals, with IC50 values of 26.58 μM and 30.31 μM, respectively. Additionally, it enhances the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione (GSH), while diminishing lactate dehydrogenase (LDH) in H2O2-treated HepG2 cells [1].

Antioxidant agent-3 (Compound 14q) exhibits good activity in DPPH radicals scavenging and ABTS + scavenging with the values of IC 50 is 26.58 μM and 30.31 μM respectively [1]. Antioxidant agent-3 shows low cytotoxicity in human normal WI-38 ( IC 50 > 100 μM) and GES ( IC 50 > 200 μM) cells [1]. Antioxidant agent-3 can enhance viability of H 2 O 2 -induced HepG2 cells [1]. Antioxidant agent-3 decreases the apoptotic percentage of HepG2 cells [1]. Antioxidant agent-3 reduces the ROS produce and LDH release, improves GSH and SOD levels in H 2 O 2 -treated HepG2 cells, and exhibits more stability in methanol solution [1]. Cell Cytotoxicity Assay [1] Cell Line: WI-38 and GES cells line Concentration: 200, 100, 50, 25 and 12.5 μM Incubation Time: 48 h Result: Showed less toxicity in hemolysis assay and weaker antiproliferative effects. Cell Viability Assay [1] Cell Line: H 2 O 2 -damaged WI-38 and HepG2 cells Concentration: 50, 25 and 12.5 μM Incubation Time: 1 h Result: Increased cells viability of H2O2-indcued cells and protected the H2O2-indcued cells against injury. Apoptosis Analysis [1] Cell Line: H 2 O 2 -damaged HepG2 cells Concentration: 50, 25 and 12.5 μM Incubation Time: 1 h Result: Protect the H 2 O 2 -injured HepG2 cells against apoptosis through antioxidant effect.