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Anti-LCAT Monoclonal Antibody
产品编号 TMAZ-0161
别名 Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase, Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase
Anti-LCAT Monoclonal Antibody is a rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody can be used in ELISA, FACS.
Anti-LCAT Monoclonal Antibody
Anti-LCAT Monoclonal Antibody
产品编号 TMAZ-0161 别名 Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase, Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase
Anti-LCAT Monoclonal Antibody is a rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody can be used in ELISA, FACS.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μg | ¥ 725¥ 1,250 | 7日内发货 | |
| 50 μg | ¥ 870¥ 1,500 | 7日内发货 | |
| 100 μg | ¥ 1,217.42¥ 2,099 | 7日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-LCAT Monoclonal Antibody is a rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody can be used in ELISA, FACS. |
| 别名 | Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase, Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase |
| Ig Type | Rabbit monoclonal IgG |
| 交叉反应 | Human |
| 验证活性 | Flow cytometry analysis of LCAT overexpressed 431F cells with TMAZ-0161, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| 应用 | ELISA, FACS |
| 推荐剂量 | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Single-pass type I membrane |
| 构建方式 | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of LCAT protein. |
| 纯化方式 | Protein A Affinity Purified |
| 性状 | Liquid |
| 缓冲液 | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 研究背景 | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed: 10329423, PubMed: 19065001, PubMed: 26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16: 0-18: 2 or 18: O-18: 2 phosphatidylcholines (PubMed: 8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed: 10722751). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed: 8016111). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (PubMed: 8016111). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (PubMed: 24620755) |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Biotin/APC/PE/AF488/AF555/AF647 |
| 可提供抗体种类 | 1 |
| 免疫原 | LCAT |
| 抗原种属 | Human |
| 基因ID | 3931 |
| Uniprot ID | |
| 研究领域 | Drug Target |
| 分子量 | 150 kDa |
存储&运输
| 储存方式 | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| 运输方式 | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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