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Anti-APOE Monoclonal Antibody
产品编号 TMAZ-0071
别名 Apolipoprotein E
Anti-APOE Monoclonal Antibody is a rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody can be used in ELISA, FACS.
Anti-APOE Monoclonal Antibody
Anti-APOE Monoclonal Antibody
产品编号 TMAZ-0071 别名 Apolipoprotein E
Anti-APOE Monoclonal Antibody is a rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody can be used in ELISA, FACS.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μg | ¥ 1,043.42¥ 1,799 | 7日内发货 | |
| 50 μg | ¥ 1,251.64¥ 2,158 | 7日内发货 | |
| 100 μg | ¥ 1,752.18¥ 3,021 | 7日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-APOE Monoclonal Antibody is a rabbit antibody targeting APOE. Anti-APOE Monoclonal Antibody can be used in ELISA, FACS. |
| 别名 | Apolipoprotein E |
| Ig Type | Rabbit monoclonal IgG |
| 交叉反应 | Human |
| 验证活性 | Flow cytometry analysis of APOE overexpressed 461F cells with TMAZ-0071, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| 应用 | ELISA, FACS |
| 推荐剂量 | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted |
| 构建方式 | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of APOE protein. |
| 纯化方式 | Protein A Affinity Purified |
| 性状 | Liquid |
| 缓冲液 | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 研究背景 | APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed: 14754908, PubMed: 1911868, PubMed: 6860692). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed: 14754908, PubMed: 1911868, PubMed: 1917954, PubMed: 23620513, PubMed: 2762297, PubMed: 6860692, PubMed: 9395455). Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed: 2762297, PubMed: 6860692, PubMed: 9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed: 1911868, PubMed: 6860692). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed: 12950167, PubMed: 1530612, PubMed: 1917954, PubMed: 20030366, PubMed: 20303980, PubMed: 2063194, PubMed: 2762297, PubMed: 7635945, PubMed: 7768901, PubMed: 8756331, PubMed: 8939961). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed: 23676495, PubMed: 7635945, PubMed: 9395455, PubMed: 9488694). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed: 1911868, PubMed: 1917954, PubMed: 23676495, PubMed: 29516132, PubMed: 9395455). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed: 2762297, PubMed: 29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed: 1917954, PubMed: 2762297, PubMed: 29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PubMed: 14754908, PubMed: 23620513, PubMed: 9395455). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PubMed: 14754908, PubMed: 23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PubMed: 9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PubMed: 25173806, PubMed: 8939961). APOE is also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells (By similarity). Binds to the immune cell receptor LILRB4 (PubMed: 30333625). APOE may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PubMed: 28111074)|(Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles (PubMed: 25122793, PubMed: 29695434). This interaction is probably promoted via the up-regulation of cellular autophagy by the virus (PubMed: 29695434) |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Biotin/APC/PE/AF488/AF555/AF647 |
| 可提供抗体种类 | 5 |
| 免疫原 | APOE |
| 抗原种属 | Human |
| 基因ID | 348 |
| Uniprot ID | |
| 研究领域 | Immunology Research |
| 分子量 | 150 kDa |
存储&运输
| 储存方式 | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| 运输方式 | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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