Acid Green 12 is a multifunctional dye used in various applications, ranging from basic scientific research to clinical diagnostics. Dyes are essential tools in biological experiments to observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. They are also widely used in traditional fields such as textile dyeing, as well as emerging fields like functional textile processing, food pigments, and dye-sensitized solar cells.