8-Bromo-ATP (8-Bromoadenosine 5'-triphosphate) is a purinergic P2X receptor agonist and an ATP analogue. It exhibits cytotoxicity towards multiple myeloma cells, with an IC50 of 23.1 μM.

8-Bromo-ATP (10-50 μM; 5 days) treatment shows cytotoxic to multiple myeloma[3]. Fluorescence measurements are made possible through the use of 8-Bromo-ATP, which selectively quenched certain tryptophan residues of the ATPase. 8-Bromo-ATP enhances the rate of dephosphorylation of native ATPase 2-3-fold when added in the absence of divalent cations[2]. Cell Viability Assay[3]Cell Line: MM 1.s cells Concentration: 10 μM, 20 μM, 30 μM, 40 μM, 50 μM Incubation Time: 5 days Result: Showed cytotoxic to multiple myeloma.