6-Hydroxybenzbromarone is the major metabolite of Benzbromarone. 6-Hydroxybenzbromarone is a protein Eyes Absent 3 (EYA3) inhibitor (IC50: 21.5 μM). It is an angiogenic agent, has strong inhibitory effects on cell migration, tubulogenesis, and angiogenic sprouting.

EYA3:21.5 μM

6-Hydroxybenzbromarone (7.5 μM; 72 hours) reduces cell proliferation by over 50%. Simultaneously, BBR and BZ treatments decrease cell viability, while other compounds tested do not affect cell viability or proliferation. 6-Hydroxybenzbromarone (7.5 μM; 1-20 hours) inhibits EC migration and tubulogenesis in HUVECs, but its impact on tube formation diminishes with high concentrations of fetal bovine serum (FBS), likely due to non-specific protein binding.

Cell Line: HUVEC cells. Concentration: 7.5 μM. Incubation Time: 72 hours