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4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) 是 4-硝基苯基 (pNP) 糖吡喃糖苷的人工底物,可用于 α-半乳糖苷酶活性的检测。它用作底物能够显著增加 pNP 的释放量。
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) 是 4-硝基苯基 (pNP) 糖吡喃糖苷的人工底物,可用于 α-半乳糖苷酶活性的检测。它用作底物能够显著增加 pNP 的释放量。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
25 mg | ¥ 132 | 现货 | |
50 mg | ¥ 178 | 现货 | |
100 mg | ¥ 247 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 150 | 现货 |
产品描述 | 4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for detecting α-galactosidase activity. The amount of released pNP is significantly increased when PNP-alpha-D-Gal is used as substrates. |
体外活性 | Using 4-nitrophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 while no activity was detected at pH 7.0. The digestion assays using pNP glycopyranosides as artificial substrates exhibit that yielding liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most. |
别名 | PNP-α-D-Gal, PNP-alpha-D-Gal, 4-硝基苯-α-D-吡喃半乳糖苷 |
分子量 | 301.25 |
分子式 | C12H15NO8 |
CAS No. | 7493-95-0 |
Smiles | OC[C@H]1O[C@H](Oc2ccc(cc2)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O |
密度 | 1.599 g/cm3 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
溶解度信息 | DMSO: 50 mg/mL (165.98 mM) | |||||||||||||||||||||||||||||||||||
溶液配制表 | ||||||||||||||||||||||||||||||||||||
DMSO
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