3-IN-PP1, a potent protein kinase D (PKD) inhibitor, exhibits pan-PKD inhibitory activity with IC50 values of 108 nM for PKD1, 94 nM for PKD2, and 108 nM for PKD3. Additionally, it serves as a broad-spectrum anticancer agent by inhibiting the growth of various tumor cells. 3-IN-PP1 is utilized in cancer research [1].

3-IN-PP1 has potent pan-PKD inhibitory activity for PKD1, PKD2 and PKD3 with IC 50 values of 108, 94 and 108 nM, respectively [1]. 3-IN-PP1 (5 μM, 0-114 h) displays potent anti-proliferative activity against PANC-1 cells [1]. 3-IN-PP1 (20 μM, 1 h) potently blocks PKD-dependent cortactin phosphorylation in PANC-1 cells [1]. 3-IN-PP1 has inhibition of different tumor cell growth with IC 50 values range from 1.6 to 39.2μM [1]. Cell Proliferation Assay [1] Cell Line: PANC-1 cell Concentration: 5 μM Incubation Time: 0-114 h Result: Significantly inhibited PANC-1 cell proliferation after incubation for 96 and 144 hours. Western Blot Analysis [1] Cell Line: PANC-1 cells Concentration: 20 μM Incubation Time: 1 h Result: Reduced cortactin phosphorylation in PANC-1 cells.