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VCAM-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 75.8 kDa and the accession number is Q3UPN1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,170 | 现货 | |
200 μg | ¥ 5,610 | 5日内发货 | |
500 μg | ¥ 11,900 | 5日内发货 |
生物活性 | Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT. |
产品描述 | VCAM-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 75.8 kDa and the accession number is Q3UPN1. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q3UPN1 |
别名 | Vcam-1,vascular cell adhesion molecule 1,CD106 |
蛋白构建 | A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus. Predicted N terminal: Phe 25 |
蛋白纯度 | > 97 % as determined by SDS-PAGE |
分子量 | 75.8 kDa (predicted); 90-100 kDa (reducing condition, due to glycosylation) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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