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TIGIT Protein, Human, Recombinant (His), Biotinylated
产品编号 TMPY-05122
TIGIT Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.2 kDa and the accession number is Q495A1-1.
TIGIT Protein, Human, Recombinant (His), Biotinylated
产品编号 TMPY-05122
TIGIT Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.2 kDa and the accession number is Q495A1-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 1,950 | 5日内发货 | |
| 100 μg | ¥ 4,620 | 5日内发货 |
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产品信息
| 生物活性 | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT His, Biotinylated at 2 μg/ml (100 μl/well) can bind Mouse CD155/PVR His & hFc , the EC50 of Mouse CD155/PVR His & hFc is 50-250 ng/mL.
2. Using the Octet RED System, the affinity constant (Kd) of TIGIT Protein, Human, Recombinant (His Tag), Biotinylated bound to MITG7192A was 0.1 nM. |
| 产品描述 | TIGIT Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 14.2 kDa and the accession number is Q495A1-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | Q495A1-1 |
| 别名 | WUCAM,VSTM3,VSIG9,T cell immunoreceptor with Ig and ITIM domains |
| 蛋白构建 | A DNA sequence encoding the TIGIT (NP_776160.2) (Met1-Phe138) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro. Predicted N terminal: Met 22 |
| 蛋白纯度 | > 90 % as determined by SDS-PAGE. |
| 分子量 | 14.2 kDa (predicted) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing Sterile PBS. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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