购物车
- 全部删除
- 您的购物车当前为空
TIGIT Dimer Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q495A1-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μg | ¥ 2,270 | 期货 | |
500 μg | ¥ 14,800 | 期货 |
生物活性 | 1. Immobilized CD155/PVR Protein, Human, Recombinant (hFc) (Cat#TMPY-00699) at 2μg/mL (100μL/well) can bind TIGIT Dimer Protein, Human, Recombinant (His) (Cat#TMPY-07043), the EC50 is 6-18 ng/mL. 2. Immobilized Anti-Tigit Antibody, Human IgG1 (Tiragolumab) at 2 μg/mL (100 μL/well) can bind TIGIT Dimer Protein, Human, Recombinant (His) (Cat#TMPY-07043), the EC50 is 1.2-4 ng/mL (Routinely tested). 3. Loaded CD155/PVR Protein, Human, Recombinant (hFc) (Cat#TMPY-00699) on ProA Biosensor, can bind TIGIT Dimer Protein, Human, Recombinant (His) (Cat#TMPY-07043) with an affinity constant of 0.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 4. CD155/PVR Protein, Human, Recombinant (hFc) (Cat#TMPY-00699) captured on Protein A chip can bind TIGIT Dimer Protein, Human, Recombinant (His) (Cat#TMPY-07043) with an affinity constant of 3.28 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). |
产品描述 | TIGIT Dimer Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q495A1-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q495A1-1 |
别名 | WUCAM,VSTM3,VSIG9,FLJ39873,DKFZp667A205 |
蛋白构建 | A DNA sequence encoding the extracellular domain of human TIGIT (NP_776160.2) (Met22-Pro141) with a C-terminal polyhistidine tag was expressed. This construct has a dimer design. |
蛋白纯度 | ≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
分子量 | 28.81 kDa (predicted); 36.72 kDa (reducing contition) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
复溶方法 | Please refer to the lot-specific COA. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy |