购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

SARS-CoV Nucleocapsid Protein (His)

产品编号 TMPY-02870

SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1.

SARS-CoV Nucleocapsid Protein (His)

SARS-CoV Nucleocapsid Protein (His)

产品编号 TMPY-02870
SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1.
规格价格库存数量
100 μg¥ 5,7505日内发货
大包装 & 定制
加入购物车
实验操作小课堂
查看更多
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。

产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
SARS-CoV Nucleocapsid Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.5 kDa and the accession number is Q6S8E1.
种属
SARS
表达系统
Baculovirus Insect Cells
标签C-His
蛋白编号Q6S8E1
别名
novel coronavirus Nucleocapsid Protein, SARS,novel coronavirus NP Protein, SARS,novel coronavirus,ncov NP Protein, SARS,cov np Protein, SARS,coronavirus Nucleoprotein Protein, SARS,coronavirus Nucleocapsid Protein, SARS,coronavirus NP Protein, SARS
蛋白构建
A DNA sequence encoding the SARS-CoV (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Met
蛋白纯度
> 80 % as determined by SDS-PAGE
分子量47.5 kDa (predicted); 47.1 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.