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Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 3,250 | 5日内发货 | |
500 μg | ¥ 13,000 | 5日内发货 | |
1 mg | ¥ 21,700 | 5日内发货 |
生物活性 | Immobilized Human Periostin, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
产品描述 | Periostin/OSF-2 Protein, Human, Recombinant (aa 22-836, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 92.1 kDa and the accession number is Q15063. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q15063 |
别名 | TRIF52,POSTN,PNRP11-412K4.1,PN,Periostin,PDLPOSTN,OSF-2,OSF2,Fasciclin I-like |
蛋白构建 | Asn22-Gln836 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 92.1 kDa (predicted). Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function. |
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