PEDF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is A0A140VKF3.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 1,950 | 5日内发货 | ||
1 mg | ¥ 19,500 | 5日内发货 |
生物活性 | Testing in progress |
产品描述 | PEDF Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 45.8 kDa and the accession number is A0A140VKF3. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | A0A140VKF3 |
别名 | serpin peptidase inhibitor, clade F,member 1, OI6, OI12, EPC-1, PEDF, PIG35 |
蛋白构建 | The Human SERPINF1 (NP_002606.3) (Met 1-Pro 418) was fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 98 % as determined by SDS-PAGE |
分子量 | 45.8 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein that has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression. |
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PEDF Protein, Human, Recombinant (His) OI 12 PIG-35 serpin peptidase inhibitor, clade F,member 1 OI-6 OI6 OI-12 PIG 35 EPC1 OI12 serpin peptidase inhibitor, clade F,member 1 EPC-1 PEDF PIG35 EPC 1 OI 6 recombinant recombinant-proteins proteins protein