NXF2 Protein, Drosophila melanogaster, Recombinant (His) is expressed in Baculovirus insect cells with C-6xHis tag. The predicted molecular weight is 33.7 kDa and the accession number is Q9VV73.

Fruit fly

Baculovirus Insect Cells

nxf2,Nuclear RNA export factor 2

QKNFLCDTGAYELVGAFLENYLREFENDEFRHNLYKYYSENSIFTLTCNYNVVQNHQTPKILQRLSKYNRHARNLRNKDYSKASDGVFFGCTYIVEILLQLPRVTHDFHSLQTDVMHYNGKGAVIYVAGLLRDEPPSTRNGHGSKTDIGGVLLGFSRQFVVTFDEANLGLGKRARRLKIANERLHITNPSKTAIRNAFSVNFPDPSERQAEEDSLDVKDHKLLLFQEVTGLISTWVTSIVEEADWDFERALKLFIQKNADHEIPDLAFA

573-841 aa

> 85% as determined by SDS-PAGE.

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

May be involved in the export of mRNA from the nucleus to the cytoplasm. In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing. On recruitment to a target transcript, interacts with single stranded RNA, thereby anchoring the complex via the nascent target transcript to chromatin and allowing Panx to recruit silencing effectors to establishing repressive heterochromatin at transposon loci. Does not affect piRNA biogenesis. The interaction with Panx stabilizes the nuclear protein complex. Does not bind nucleoporins, but regulates sbr/Nxf1 binding to nucleoporins and, indirectly, transposon exports.