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NOV/CCN3 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 37 kDa and the accession number is A0A024R9J4.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
100 μg | ¥ 5,750 | 5日内发货 | |
1 mg | ¥ 37,500 | 5日内发货 |
生物活性 | Measured by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cells. When cells are added to CCN3-coated plates (10 μg/ml, 100 μl/well), >50% cells will adhere specifically after 60 minutes at 37 ℃. |
产品描述 | NOV/CCN3 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 37 kDa and the accession number is A0A024R9J4. |
种属 | Human |
表达系统 | Baculovirus Insect Cells |
标签 | C-His |
蛋白编号 | A0A024R9J4 |
别名 | IGFBP-9,CCN3,IGFBP9,NOVh,IBP-9,nephroblastoma overexpressed |
蛋白构建 | The full length of Human NOV (NP_002505.1) (Met 1-Met 357) was expressed, fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 | > 94 % as determined by SDS-PAGE |
分子量 | 37 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10 mM Imidazole, 10% Glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Protein NOV homolog, also known as Nephroblastoma-overexpressed gene protein homolog, NOV, and CCN3, is a putative ligand for integrin receptors, is tightly associated with the extracellular matrix, and mediates diverse cellular functions, including cell adhesion and proliferation. CCN3 has been shown to negatively regulate growth although it promotes migration in a cell type-specific manner. This secreted protein belongs to the CCN family, and its expression was observed in a broad variety of tissues from the early stage of development, and altered expression of CCN3 has been observed in a variety of tumors, including hepatocellular carcinomas, Wilm's tumors, Ewing's sarcomas, gliomas, rhabdomyosarcomas, and adrenocortical carcinomas. Mature CCN3 protein has five distinct modules and truncated protein variants with altered function are found in many cancers. CCN3 acts through the core stem cell signaling pathways including Notch and Bone Morphogenic Protein, connecting CCN3 with the modulation of self-renewal and maturation of some cell lineages including hematopoietic, osteogenic, and chondrogenic. CCN3 may affect the extracellular environment of the niche for hematopoietic stem cells. CCN3 has emerged as a key player in stem cell regulation, hematopoiesis, and a crucial component within the bone marrow microenvironment. |
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