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MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated

产品编号 TMPK-01397

MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33 kDa and the accession number is A0A2K5TW94-1.

MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated

MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated

产品编号 TMPK-01397
MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33 kDa and the accession number is A0A2K5TW94-1.
规格价格库存数量
100 μg¥ 5,1805日内发货
500 μg¥ 20,7005日内发货
1 mg¥ 34,6005日内发货
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生物活性

生物活性
Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus MSLN, His Tag with the EC50 of 54.8ng/ml determined by ELISA.
产品描述
MSLN/Mesothelin Protein (Primary Amine Labeling), Cynomolgus, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 33 kDa and the accession number is A0A2K5TW94-1.
种属
Cynomolgus
表达系统
HEK293 Cells
标签C-His
蛋白编号A0A2K5TW94-1
别名
MPFSMRP,Mesothelin,MPF,SMR,MSLN,CAK1
蛋白构建
Asp296-Gly580
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量33 kDa (predicted). Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

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