LIFR Protein, Rat, Recombinant (His)

产品编号 TMPY-03306

LIFR Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 90 kDa and the accession number is G3V7K2.

LIFR Protein, Rat, Recombinant (His)

产品编号 TMPY-03306

LIFR Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 90 kDa and the accession number is G3V7K2.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

生物活性

生物活性	1.Measured by its binding ability in a functional ELISA. Immobilized Mouse LIF hFc at 2 μg/ml (100 μl/well) can bind Rat LIFR His , the EC50 of Rat LIFR His is 300-700 ng/mL. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant human LIF.
产品描述	LIFR Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 90 kDa and the accession number is G3V7K2.
种属	Rat
表达系统	HEK293 Cells
标签	C-His
蛋白编号	G3V7K2
别名	leukemia inhibitory factor receptor α,leukemia inhibitory factor receptor alpha
蛋白构建	A DNA sequence encoding the rat LIFR (G3V7K2) (Met1-Ser829) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 44
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	90 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

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