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Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. Human coronavirus HKU1 (isolate N5) Protein I (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.8 kDa and the accession number is Q0ZME2.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μg | ¥ 1,320 | 20日内发货 | |
100 μg | ¥ 2,470 | 20日内发货 | |
1 mg | ¥ 10,700 | 20日内发货 |
生物活性 | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
产品描述 | Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. Human coronavirus HKU1 (isolate N5) Protein I (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.8 kDa and the accession number is Q0ZME2. |
种属 | HCoV-HKU1 |
表达系统 | E. coli |
标签 | N-10xHis, C-Myc |
蛋白编号 | Q0ZME2 |
别名 | Protein I,N internal ORF protein,Accessory protein N2,N,Orf8 protein,Protein in nucleocapsid ORF |
氨基酸序列 | MLEVEAPLEIVQESSRKLLGLTNLSEAIKPIIEAENPNQNSLCLLNHKETLSHIIPGSLGLPNFKKVETLNFQMVKEYPLLTGYPLLKQKDIGINTTGVLLKQLMVNKSSCYQDGISTISVPVHMPVHPMVMPTKVSSGSLVTKLILLFPPMFRQGILLFKKLSLLGFRLVRFCLKAIMLKAQEGLLLIAGQVHVLNHVDPIIVH |
蛋白构建 | 1-205 aa |
蛋白纯度 | > 85% as determined by SDS-PAGE. |
分子量 | 27.8 kDa (predicted) |
缓冲液 | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Structural protein that is not essential for the viral replication either in tissue culture or in its natural host. |
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