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GPA33/A33 Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-01147

GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.

GPA33/A33 Protein, Human, Recombinant (His & Avi)

GPA33/A33 Protein, Human, Recombinant (His & Avi)

产品编号 TMPK-01147
GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.
规格价格库存数量
100 μg¥ 2,2105日内发货
500 μg¥ 8,9005日内发货
1 mg¥ 14,8005日内发货
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生物活性

生物活性
1. Immobilized Human GPA33, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. 2. Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay.
产品描述
GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号Q99795-1
别名
A33,Glycoprotein A33,MGC129986,GPA33,MGC129987
蛋白构建
Ile22-Val235
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量26.5 kDa (predicted). Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.

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