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DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated

产品编号 TMPK-00157

Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.

DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated

DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated

产品编号 TMPK-00157
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.
规格价格库存数量
100 μg¥ 5,1805日内发货
500 μg¥ 20,7005日内发货
1 mg¥ 34,6005日内发货
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产品信息

生物活性
Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA.
产品描述
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. DKK1 C terminal Domain Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 38.69 kDa and the accession number is O94907.
种属
Human
表达系统
HEK293 Cells
标签C-hFc-Avi
蛋白编号O94907
别名
SK,hDkk-1,hDkk1,Dkk-1,Dkk1,Dickkopf-related 1,Dickkopf-1
蛋白构建
Met178-His266
蛋白纯度
> 95% as determined by Tris-Bis PAGE
分子量38.69 kDa (predicted). Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM NaAc, 150 mM NaCl (pH 5.0). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.

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