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Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae. Cry2Aa Protein, Bacillus thuringiensis subsp., Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 29.7 kDa and the accession number is P0A377.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μg | ¥ 2,290 | 20日内发货 | |
100 μg | ¥ 4,320 | 20日内发货 | |
1 mg | ¥ 14,600 | 20日内发货 |
生物活性 | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
产品描述 | Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae. Cry2Aa Protein, Bacillus thuringiensis subsp., Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 29.7 kDa and the accession number is P0A377. |
种属 | Bacillus thuringiensis subsp. kurstaki |
表达系统 | E. coli |
标签 | N-10xHis, C-Myc |
蛋白编号 | P0A377 |
别名 | Pesticidal crystal protein Cry2Aa,P2 crystal protein,Mosquito factor,Insecticidal delta-endotoxin CryIIA(a),Crystaline entomocidal protoxin,cryIIA(a),cryII,cryB1,cry2Aa,71 kDa crystal protein |
氨基酸序列 | LMVSSGANLYASGSGPQQTQSFTAQNWPFLYSLFQVNSNYILSGISGTRLSITFPNIGGLPGSTTTHSLNSARVNYSGGVSSGLIGATNLNHNFNCSTVLPPLSTPFVRSWLDSGTDREGVATSTNWQTESFQTTLSLRCGAFSARGNSNYFPDYFIRNISGVPLVIRNEDLTRPLHYNQIRNIESPSGTPGGARAYLVSVHNRKN |
蛋白构建 | 267-472 aa |
蛋白纯度 | > 90% as determined by SDS-PAGE. |
分子量 | 29.7 kDa (predicted) |
缓冲液 | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae. |
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