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CD99L2 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01669

CD99L2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.3 kDa and the accession number is Q8BIF0-2.

CD99L2 Protein, Mouse, Recombinant (His)

CD99L2 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01669
CD99L2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.3 kDa and the accession number is Q8BIF0-2.
规格价格库存数量
100 μg
¥ 3,820
5日内发货
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产品信息

生物活性
Measured by its ability to bind biotinylated recombinant mouse CD99L2 in functional ELISA.
产品描述
CD99L2 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 16.3 kDa and the accession number is Q8BIF0-2.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号Q8BIF0-2
别名
Xap89,Mic2l1,CD99 molecule-like 2,AW548191
蛋白构建
A DNA sequence encoding the mouse CD99L2 (NP_612182.1) extracellular domain (Met 1-Ala 164) was expressed, fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 26
蛋白纯度
> 98 % as determined by SDS-PAGE
分子量16.3 kDa (predicted); 30-40 kDa (reducing condition, due to glycosylation)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
CD99 antigen-like protein 2, also known as MIC2-like protein 1, CD99L2 and MIC2L1, is a single-pass type I membrane protein which belongs to theCD99 family. CD99L2 is expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression of CD99L2 is seen in thymus. It is also expressed in E18 uterus and placenta. CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-alpha, where the need for PECAM-1 is known to be bypassed. CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane. CD99L2 may function as a homophilic adhesion molecule. It functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. CD99L2 does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.

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