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CCR8 Protein-Nanodisc, Human, Recombinant (Flag-Strep & His) is expressed in HEK293 Cells. The accession number is P51685.
规格 | 价格 | 库存 | 数量 |
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生物活性 | 1. Immobilized CCR8 Protein-Nanodisc, Human, Recombinant (Flag-Strep & His) (Cat#TMPY-07066) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody1, Human IgG1, the EC50 is 1-5 ng/mL (QC tested). 2. Immobilized CCR8 Protein-Nanodisc, Human, Recombinant (Flag-Strep & His) (Cat#TMPY-07066) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody2, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested). 3. Immobilized CCR8 Protein-Nanodisc, Human, Recombinant (Flag-Strep & His) (Cat#TMPY-07066) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody3, Human IgG1, the EC50 is 3-10 ng/mL (Routinely tested). |
产品描述 | CCR8 Protein-Nanodisc, Human, Recombinant (Flag-Strep & His) is expressed in HEK293 Cells. The accession number is P51685. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-Flag-Strep, C-His |
蛋白编号 | P51685 |
蛋白构建 | A DNA sequence encoding the Human CCR8 (P51685)(Met1-Cys228) & (Asn230-Ser318) was expressed and linked by a Fusion Protein, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.This protein was prepared using our Nanodisc platform.
Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment. |
蛋白纯度 | ≥ 85% as determined by SDS-PAGE. |
分子量 | 46.51 kDa (predicted); 96.9 kDa (dimer) and 44 kDa (reducing contition) |
内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 50 mM HEPES, 150 mM NaCl, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
存储 | Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 | It is supplied and shipped as liquid with dry ice. |
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