Bovine coronavirus (strain Mebus) Non-structural Protein 4b (His) is expressed in yeast with C-6xHis tag. The predicted molecular weight is 6.3 kDa and the accession number is P22052.

BCoV

P. pastoris (Yeast)

ns4.8,Non-structural protein of 4.8 kDa,4.8 kDa accessory protein

MPMATTIDGTDYTNIMPSTVSTTVYLGCSIGIDTSTTGFTCFSRY

1-45 aa

> 90% as determined by SDS-PAGE.

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.