Beta Klotho Protein, Human, Recombinant (His)

Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites. Beta Klotho Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 111.6 kDa and the accession number is Q86Z14.

规格	价格/CNY		数量
100 μg	￥ 10,300	5日内发货
500 μg	￥ 41,500	5日内发货
1 mg	￥ 69,200	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 10,300

5日内发货

500 μg

￥ 41,500

5日内发货

1 mg

￥ 69,200

5日内发货

生物活性

1. Immobilized Human FGF21, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA. 2. Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay.

产品描述

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q86Z14
别名	beta-klotho, MGC142213, betaKlotho, BKL, β-klotho, KLB, klotho beta like, Klotho β, βKlotho, β Klotho, klotho β like, Klotho beta
蛋白构建	Met30-Thr983
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	111.6 kDa (predicted). Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.