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ASAM Protein, Mouse, Recombinant (His)

产品编号 TMPY-01698

别名: ACAM, AW557819, CXADR-like membrane protein, 9030425E11Rik, ASP5

ASAM Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is Q8R373-1.

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ASAM Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货

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参考文献

生物活性	Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to mouse ASAM coated plates (30 μg/ml, 100 μl/well), approximately >40 % will adhere after one hour at 37 ℃.
产品描述	ASAM Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.6 kDa and the accession number is Q8R373-1.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q8R373-1
别名	ACAM, AW557819, CXADR-like membrane protein, 9030425E11Rik, ASP5
蛋白构建	The extracellular domain of Mouse ASAM (Q8R373-1) (Met 1-Met 232) was expressed, with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE
分子量	25.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Adipocyte-specific adhesion molecule (ASAM), also known as ACAM and CLMP, is a type I transmembrane protein and a member of the CTX (cortical thymocyte marker in Xenopus) family within the immunoglobulin superfamily. ASAM protein is highly expressed in the small intestine and placenta, and is found at intermediate levels in the heart, skeletal muscle, colon, spleen, kidney, and lung, and appears in low levels in the liver and peripheral blood leukocytes as well. ASAM is a transmembrane component of tight junctions in epithelial cells that can mediate cell aggregation and regulate transepithelial resistance across polarized epithelial cells. In addition, its expression is strongly correlated with white adipose tissue (WAT) mass of human and rodents with obesity.

参考文献

1. Eguchi J, et al. (2005) Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity. Biochem J. 387(Pt 2): 343-53. 2. Sze KL, et al. (2008) Expression of CLMP, a novel tight junction protein, is mediated via the interaction of GATA with the Kruppel family proteins, KLF4 and Sp1, in mouse TM4 Sertoli cells. J Cell Physiol. 214(2): 334-44. 3. Sze KL, et al. (2008) Post-transcriptional regulation of CLMP mRNA is controlled by tristetraprolin in response to TNFalpha via c-Jun N-terminal kinase signalling. Biochem J. 410(3): 575-83.

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Keywords

ASAM Protein, Mouse, Recombinant (His) ACAM AW557819 AW 557819 ASP 5 CXADR-like membrane protein 9030425E11Rik ASP-5 ASP5 AW-557819 recombinant recombinant-proteins proteins protein

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