RFRP-3, mouse is a functional analog of avian Gonadotropin-Inhibitory Hormone (GnIH) that binds to GPR147. It reduces progesterone synthesis by inhibiting FSHR and key steroidogenic enzymes (P450scc, 3β-HSD, StAR). RFRP-3, mouse induces apoptosis by increasing p53 and can inhibit the ERK signaling pathway. Additionally, RFRP-3 is applicable in follicle development research.

RFRP-3 (10-1000 ng/mL, 24 h) 能显著减少小鼠卵巢颗粒细胞内 Progesterone 的分泌，但对 Estradiol 的分泌无影响。同时，RFRP-3 抑制类固醇生成过程和 FSHR 的表达（抑制 p450scc、3β-HSD、StAR 和 FSHR）。此外，RFRP-3 诱导细胞凋亡，中等剂量下降低 Bcl-2 蛋白表达，增加 p53 蛋白水平，并抑制 ERK 信号通路（p-ERK1/2 蛋白）。