LasR-IN-2, a compound that inhibits the activity of LasR, forms hydrogen bonding with the TRY-56 residue. It finds application in the study of bacterial infection, neutropenia, severe burns, and chronic lung disease in cystic fibrosis (CF) [1].

LasR-IN-2 (Compound 8a, 4.68-150 μg/mL, 24 h) inhibits P. aeruginosa growth [1]. LasR-IN-2 (18.5 μM, 24 h) inhibits biofilm formation, pyocyanin production, and rhamnolipids production [1]. LasR-IN-2 (24 h) inhibits human dermal fibroblasts (HDFa) growth with an IC 50 value of 102 μM [1]. Cell Viability Assay [1] Cell Line: P. aeruginosa (ATCC27853) Concentration: 4.68-150 μg/mL Incubation Time: 24 h Result: Inhibited bacterial growth with a MIC value of 74.40 μM.