HKPerox-2 is an excellently selective and sensitive green fluorescent probe toward H 2 O 2 over 30-fold other tested ROS/RNS in chemical and biological systems. HKPerox-2 is a O-methyl rhodol derivative and specifically recognize H 2 O 2 based on a tandem payne/dakin reaction.

HKPerox-2 is a O-methyl rhodol derivative and reaches a plateau within 30 min upon treatment of 100 μM H 2 O 2 , and the rate constants k obs is determined to be 2.0x10 -2 s for HKPerox2[1].HKPerox-2 (0-20 μM; 24 hours) is nontoxic to cells at working concentrations in RAW264.7 macrophages[1].

HKPerox-2 should be co-incubated with 100 μM of trichloroacetonitrile (CCl3CN) for H 2 O 2 detection and molecular imaging. DMSO should be avoided during H 2 O 2 detection, as DMSO could scavenge activated H 2 O 2 in the presence of CCl3CN . DMF is the recommended solvent to prepare probe stock solution.