GSD-11 inhibits the Akt/mTOR signaling pathway. GSD-11 inhibits the PANC-1 cells migration and colony formation. GSD-11 is a potent and selective anti-austerity agent that has the potential for the pancreatic cancer research [1].

GSD-11 (0,1, 10, 100, 1000 μM) shows potent cytotoxicity against PANC-1 cells with PC 50 value of 0.72 μM in nutrient-deprived medium (NDM), and IC 50 value of 3.5 μM in Dulbecco’s modified Eagle’s medium (DMEM) [1]. GSD-11 (10 μM, 24 h) induces morphological change in PANC-1 cells [1]. GSD-11 (3, 5 μM, 24 h) inhibits the migration of PANC-1 tumor cells [1]. GSD-11 (1, 3, 5 μM, 24 h) shows strong colony formation inhibitory effect in PANC-1 tumor cells [1]. GSD-11 (2.5, 5, 10 μM) inhibits the survival of PANC-1 cells through the inhibition of the Akt/mTOR pathway under NDM conditions [1]. Cell Cytotoxicity Assay [1] Cell Line: PANC-1 cells Concentration: 0,1, 10, 100, 1000 μM Incubation Time: Result: Showed preferential cytotoxicity against PANC-1 cells with PC 50 of 0.72 μM in nutrient-deprived medium (NDM), and IC 50 value of 3.5 μM in Dulbecco’s modified Eagle’s medium (DMEM). Western Blot Analysis [1] Cell Line: PANC-1 cells Concentration: 2.5, 5, 10 μM Incubation Time: Result: Inhibited the survival of PANC-1 cells through the inhibition of the Akt/mTOR pathway under NDM conditions.