COX-2/PI3K-IN-2 (compound 5f) has properties of anti-inflammatory and anti-cancer. COX-2/PI3K-IN-2 is a selective inhibitor of COX-2 (Ki = 3.02 nM) as well as a potent inhibitor of PI3K (IC 50 = 2.78 nM) [1].

COX-2/PI3K-IN-2 (compound 5f) (50-500 μg/mL, 72 hours) exerts potent antitumor activities against Breast cancer (MCF-7) cells [1]. COX-2/PI3K-IN-2 (compound 5f) (10-150 μg/mL) has antioxidant properties with H 2 O 2 scavenging activity equivalent to ascorbic acid and higher lysozyme inhibition ( IC 50 =3.25 nM) [1]. Cell Cytotoxicity Assay [1] Cell Line: Breast cancer (MCF-7) cells and normal breast epithelial (MCF-10A) cells Concentration: 50–500 μg/mL Incubation Time: 72 hours Result: Caused 40 % cell death at 1.65 μM in MCF-7 cells.