BMP Agonist 1 (compound 2 b) is a small-molecule stimulator of bone morphogenic protein (BMP) signaling, crucial for the differentiation of C2C12 cells. It acts by inhibiting GSK3β and enhancing β-catenin signaling, thereby synergistically modulating Id2 and Id3 expression. This compound is employed in skeletal disease and defect research [1].

BMP促效剂1在0.01-10μM浓度范围内，处理24小时，能显著降低C2C12细胞增殖[1]。在0.5-1μM浓度，2小时处理后，该化合物抑制C2C12细胞内的GSK3β，并增强了β-catenin的信号表达，同时协调促进Id2和Id3表达[1]。此外，BMP促效剂1在0.01-10μM浓度下与Chromenone 1（0.5−1μM）共同作用，有效地促进了C2C12细胞内BMP典型增强因子Chromenone 1所介导的成骨分化（Alp活性）[1]。