Benzo[a]pyrene (3,4-Benzopyrene) shows lung carcinogenicity in mice in a dose-dependent manner.

在雌性A/J小鼠中，由Benzo[a]pyrene（B[a]P）引发的肺部肿瘤发生具有剂量依赖性。与对照组相比，接受0.25、0.50和1.0 mg B[a]P治疗的雌性小鼠的增生值发生率显著增高。接受1.0 mg B[a]P治疗的雌性小鼠中腺瘤的发生率显著高于对照组。接受0.50或1.0 mg B[a]P的雌性小鼠增生的多发性显著高于对照组。接受1.0 mg B[a]P治疗的组中腺瘤的多发性也显著高于对照组。B[a]P以剂量依赖的方式显著增加雌性A/J小鼠增生和腺瘤的发生率。肺部增生性病变被归类为支气管肺泡增生或腺瘤，未观察到恶性肿瘤（腺癌）[1]。

After a 1- or 2-week acclimatization period, 360 adult male A/J mice and 520 adult female A/J mice were used for the experiment.?Mice were allocated, using a body weight-based randomization process, to a total of 22 groups.?Briefly, each animal received a single intraperitoneal administration of one of the initiators, at one of the indicated doses, on day 1.?The animals were observed daily for clinical signs and mortality, and body weights were measured weekly.?Following an overnight fast at 26 weeks after dosing, all mice were anesthetized with sevoflurane and weighed.?They were then sacrificed by exsanguination from the abdominal aorta and caudal vena cava and subjected to necropsy[1].