2-Deoxy-2-fluoro-L-fucose is a fucosylation inhibitor that inhibits the de novo synthesis of GDP-fucose in mammalian cells, effectively inhibits the proliferation of human cancer and primary endothelial cells, and has potential as an angiogenic therapeutic agent. It also inhibits fucosylation of plant cell wall polysaccharides, particularly affecting the dimerization of the pectin-like polysaccharide RG-II, thereby inhibiting root growth and cell elongation.2-Deoxy-2-fluoro-L-fucose also reduces the phosphorylation of Smad 1/5 and Smad 2 in 4T1 cells.

方法：2-Deoxy-2-fluoro-L-fucose （100，250，500 μM）处理 4T1 细胞，裂解物的 Aleuria aurantia 凝集素 （AAL）用WesternBlot 检测。
结果：2-Deoxy-2-fluoro-L-fucose 降低了所有浓度的 AAL 信号并抑制了岩藻糖基化。
方法：2-Deoxy-2-fluoro-L-fucose （100 μM，48小时）处理 4T1 细胞，然后接种活细胞通过迁移测定进行分析；评估 Smad 蛋白，评估调节迁移的信号分子变化。
结果：2-Deoxy-2-fluoro-L-fucose 处理的细胞的迁移能力受损并降低了 Smad 1/5 和 Smad 2 的磷酸化。[1]