Serpin D1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01254

Serpin D1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 53.5 kDa and the accession number is P49182.

Serpin D1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01254

Serpin D1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 53.5 kDa and the accession number is P49182.

规格	价格	库存	数量
50 μg	¥ 3,820	5日内发货

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产品信息

生物活性	Measured by its ability to inhibit thombin cleavage of fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is < 1.2 nM.
产品描述	Serpin D1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 53.5 kDa and the accession number is P49182.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P49182
别名	Serpind1,serpin peptidase inhibitor, clade D (heparin cofactor), member 1,MGC107662,HCII,Hcf2,AI303446,AI30344 6,AA985900
蛋白构建	A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag. Predicted N terminal: Glu 24
蛋白纯度	> 98 % as determined by SDS-PAGE
分子量	53.5 kDa (predicted); 65-70 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM HEPES, 150 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	SerpinD1, also known as heparin cofactor II, is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.